Professor Haruko Ogawa of the Veterinary Medicine Research Department, Obihiro University of Agriculture and Fisheries, Japan, and Yohei Takeda, Special Assistant Professor, Global Pesticide Research Center, and others. A research team was formed to demonstrate the inactivating effect of Hypochlous acid water on a novel coronavirus (SARS-CoV-2). In addition to the first reported pH of 2.5 and free effective chlorine (FAC) concentration of 64 mg on May 14, 2020. In addition to the results for water with Hypochlous acid water (EW) above /L, This time, the inactivation effect of water with higher pH and lower FAC was also verified for Hypochlous acid water.
The research team first assessed the inactivation of SARS-CoV-2 by Hypochlous acid water at different pH and FAC.The research team prepared a pH of 2.5 (FAC of 74 mg/L), a pH of 4.5 (FAC of 45 mg/L) and three Hypochlous acid waters at pH 6.0 (29 mg/L for FAC),experimentally mix the viral liquid with the above-mentioned Hypochlous acid water at a ratio of 1:9 and allow it to react for 10 minutes or 1 minute at room temperature. Virus titers (i.e., "infectious residual virus load") were then calculated using the TCID50 method.For comparison with Hypochlous acid water, the research team also prepared a control group of sterile distilled water (DDW) without virucidal activity mixed with virus phase.
The experimental results showed that Hypochlous acid water at pH 2.5 (FAC 74 mg/L) in a reaction time of 1 minute wouldVirus inactivated below detection limit; pH 4.5 (45 mg/L FAC) and pH 6.0 (45 mg/L FAC). (29 mg/L) of Hypochlous acid water inactivated more than 99% of the virus, but the residual viral load was not reduced below the detection limit. Infectious virus was still present. In addition, at a reaction time of 10 minutes, pH 4.5 (45 mg/L FAC) and pH 6.0 (45 mg/L FAC) were observed. for 29 mg/L) did not change much from the degree of viral inactivation at a reaction time of 1 min,The virus has still not fallen below detection limits, The virus has still not fallen below detection limits (Figure 1).
Next, the research team mixed the viral solution with Hypochlous acid water at pH 4.5-6.0 in a 1:15 ratio. The amount of residual virus after 1 min of response was assessed.
The results showed that a pH of 4.5 (45 mg/L FAC) and a pH of 6.0 (29 mg FAC) /L) of Hypochlous acid water if used up to 15 times the amount of viral liquid,can inactivate more than 99.9% of the virus within a 1-minute reaction time, reducing it below the detection limit (Figure 2).
In addition to these results, the research team also confirmed that for viral fluids containing large amounts of protein, to use hypochlorous acid with a pH of 4.5 Water (45 mg/L FAC) and pH 6.0 Hypochlous acid water (29 mg/L FAC) to achieve sufficient Virus inactivation requires a greater amount of aqueous Hypochlous acid water solution (Depending on the situation, an aqueous Hypochlous acid water solution 40 times greater than the viral solution may have to be used).
The results of this study showed that Hypochlous acid water at pH 4.5-6.0 (FAC 45-29 mg/L) had a positive effect on Although the novel coronavirus is inactivated, its inactivation is less effective than at pH 2.5 (FAC of 64 mg/L and above). of Hypochlous acid water. However, Hypochlous acid water with a pH of 4.5-6.0 (FAC of 45-29 mg/L), if used in large quantities, can also be Powerfully inactivates the virus in a short time, such as 1 minute.
In this study, weakly acidic and slightly acidic Hypochlous acid water with lower concentrations of free chlorine was also found to have lower concentrations of free chlorine than the previously reported strongly acidic Hypochlous acid water. It has some inactivating effect on novel coronaviruses. However, it is less active than strongly acidic Hypochlous acid water, so cleaning fingers and contaminated parts to adequately remove contamination requires the use of large amounts of of Hypochlous acid water to clean, or perform multiple washings.
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